Search results for "Living cell"
showing 9 items of 9 documents
Ein neuer weg zur herstellung von oligomeren aus acryl- und vinylverbindungen; modell einer matrizenreaktion
1966
In einer Reihe vorangehender Untersuchungen wurde die Herstellung molekulareinheitlicher, phenolischer Mehrkernverbindungen beschrieben. Sie besitzen je Phenolbaustein eine phenolische Hydroxylgruppe, wie systematisch untersuchte Acylierungen sowie Methylierung und Arylierung dieser Mehrkernverbindungen zeigen. In der vorliegenden Mitteilung wurde bei einer Zwei- und Dreikernverbindung an den phenolischen Hydroxylgruppen mit Acrylsaurechlorid umgesetzt, so das die entstandenen Verbindungen so viele polymerisationsfahige Reste wie phenolische Bausteine besasen. Die Polymerisation der Acrylsaurederivate in sehr groser Verdunnung gab oligomere Leiterverbindungen, die durch alkalische Hydrolyse…
How self-assembly of amphiphilic molecules can generate complexity in the nanoscale
2015
Abstract Given the importance of nanomaterials and nanostructures in modern technology, in the past decades much effort has been directed to set up efficient bottom up protocols for the piloted self-assembly of molecules. However, molecules are generally disinclined to adopt the desired structural organization because they behave according to their own specific intermolecular interactions. Thus, only some selected classes of chemical compounds are capable to lead to useful self-assembled structures. Amphiphiles, simultaneously possessing polar and apolar moieties within their molecular architecture, can give a wide scenario of possible intermolecular interactions: polar–polar, polar–apolar,…
Electromagnetic Radiation, a Living Cell and the Soul: A Collated Hypothesis
2015
The soul is believed to be an immortal essence of living things in scores of philosophical and religious traditions but sparsely understood by science. The word ‘soul’ does not have a scientific definition but through this paper is hypothesized to be an indefinite, non-structured, massless energy made up of electromagnetic radiations that is confined in the cytoskeletal network of the biological cell. Electromagnetic radiations continually interact with the biological cell and propagate within the cell; by a pathway known as ‘Cell-Soul Pathway’. This pathway is a coherent, imperceptible, uncontainable and recyclable support pathway, which uses this energy to promulgate consciousness in a bi…
A reliable and unbiased human protein network with the disparity filter
2017
AbstractThe living cell operates thanks to an intricate network of protein interactions. Proteins activate, transport, degrade, stabilise and participate in the production of other proteins. As a result, a reliable and systematically generated protein wiring diagram is crucial for a deeper understanding of cellular functions. Unfortunately, current human protein networks are noisy and incomplete. Also, they suffer from both study and technical biases: heavily studied proteins (e.g. those of pharmaceutical interest) are known to be involved in more interactions than proteins described in only a few publications. Here, we use the experimental evidence supporting the interaction between protei…
Off–on BODIPY-based chemosensors for selective detection of Al3+ and Cr3+versus Fe3+ in aqueous media
2014
[EN] Two new off-on BODIPY-based chemosensors that are highly sensitive for trivalent cations in aqueous solutions are described. Compound 2 exhibits selective sensing of Al3+ and Cr3+ versus Fe3+ through two different channels (UV-vis and fluorescence).
ISWI Regulates Higher-Order Chromatin Structure and Histone H1 Assembly In Vivo
2007
Imitation SWI (ISWI) and other ATP-dependent chromatin-remodeling factors play key roles in transcription and other processes by altering the structure and positioning of nucleosomes. Recent studies have also implicated ISWI in the regulation of higher-order chromatin structure, but its role in this process remains poorly understood. To clarify the role of ISWI in vivo, we examined defects in chromosome structure and gene expression resulting from the loss of Iswi function in Drosophila. Consistent with a broad role in transcriptional regulation, the expression of a large number of genes is altered in Iswi mutant larvae. The expression of a dominant-negative form of ISWI leads to dramatic a…
In situ formation of pyronin dyes for fluorescence protease sensing
2017
International audience; We report a reaction-based strategy for the fluorogenic detection of protease activity. Based on the "covalent-assembly" probe design principle recently put forward by the Yang group for detection of Sarin related threats (J. Am. Chem. Soc., 2014, 136, 6594-6597), we have designed two unusual nonfluorescent caged precursors (mixed bis-aryl ethers) which are readily converted into a fluorescent unsymmetrical pyronin dye through a domino cyclisation-aromatisation reaction triggered by penicillin G acylase (PGA) or leucine aminopeptidase (LAP). Fluorescence-based in vitro assays and HPLCfluorescence/- MS analyses support the claimed activation mechanism whose the furthe…
Infrared microspectroscopy of biochemical response of living cells in microfabricated devices
2010
Abstract First experiments demonstrating the suitability of novel microfabricated fluidic devices for measuring living cells in physiological environment by synchrotron radiation (SR) Fourier Transform Infrared microspectroscopy (μ-FTIR) are presented. The devices were fabricated on CaF 2 windows, using the photoresist XARP 3100/10 to define the liquid cell lay-out. Therefore, the sample holder is transparent to both visible and infrared light, robust, completely recyclable and with a precise spacing. Using prototype devices of thicknesses 9, 5 and 3 μm, we studied the response of the U937 monocytic cell line to mechanical compression. The temporal evolution of the FTIR spectra, characteris…
Ex Vivo Tracking of Endogenous CO with a Ruthenium(II) Complex.
2017
[EN] A two-photon fluorescent probe based on a ruthenium(II) vinyl complex is capable of selectively detecting carbon monoxide in cells and ex vivo using mice with a subcutaneous air pouch as a model for inflammation. This probe combines highly selective and sensitive ex vivo detection of endogenous CO in a realistic model with facile, inexpensive synthesis, and displays many advantages over the widely used palladium-based systems.